Species specific PCR based detection of Escherichia coli from Indian foods
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چکیده
منابع مشابه
Species specific PCR based detection of Escherichia coli from Indian foods
Escherichia coli is a faecal indicator and certain virotypes are known as pathogens. Therefore, detection and prevention of E. coli in food is very important. The existing rapid methods concentrate on detecting the pathogenic E. coli instead of total E. coli population. Present study evaluates the use of two molecular markers (uidA and flanking region of uspA) specific for the E. coli in combin...
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BACKGROUND Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. METHODS We developed fluorescent amplification-based specific hybridization (FLASH-PCR) assay to detect the Stx-encoding gene Stx-1 of E. coli O157:H7. RESULT PCR product of 336 bp was...
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ژورنال
عنوان ژورنال: 3 Biotech
سال: 2017
ISSN: 2190-572X,2190-5738
DOI: 10.1007/s13205-017-0784-8